T-cell proliferation in response to the stimulations of PLP peptide and KLH in control and MPL-PURO–transferred mice.
MPL-PURO–treated and untreated control mice were primed with PLP p139-151/CFA or KLH/CFA in the hind footpads, and the draining LNs were removed 9 days later. Doses of 4 × 105 LN cells were cultured in vitro with or without the presence of the specific antigens. 3H-thymidine was added 24 hours after incubation, and cells were harvested 10 hours later. The data show mean ± SD CPM derived from triplicate cultures. Responses to PLP p139-151 are shown in (A) and to KLH in (B). Each bar represents one mouse; the black bars represent the untreated control mice, and the gray ones represent MPL-PURO–treated mice. Concentrations of the stimulating antigen are indicated in the x-axis.
