Relative quantitation of the TC transcripts in HT-29 cells.

Separate amplifications were performed of a truncated TC cDNA fragment encoding for a protein containing either P or R at codon 259. In cycle No. 24, the TC transcript 259-R reaches an intensity 2.6 times weaker than the TC transcript 259-P variant. This result was observed repeatedly in 3 experiments and indicated that the discrepancy between the TC gene products could be explained by a mechanism occurring at the transcriptional level. OD indicates optical density.