Direct detection of FVIII and autoantibody immune complexes in plasma.

Each FVIII band was detected by using mAb NMC-VIII/10 (A), which recognizes the 80-kd LCh but not the 72-kd LCh, or mAb JR8 (B), which recognizes the 44-kd HCh. Th (−) represents untreated material and Th (+) represents the material treated with thrombin. Type 1 autoantibody plasma was used as a control.