VEGF induces cell migration of human peripheral monocytes and macrophagelike cells differentiated from cord blood cells.

(A) Human peripheral blood monocytes were analyzed for VEGF-dependent cell migration. Experimental procedures are outlined in detail in “Materials and methods.” In some wells, anti–Flt-1 neutralizing mAb KM1732 (1 μg/mL) was used for the blocking of Flt-1. (B) Human macrophagelike cells (adherent cells) were obtained from CD34⁺ cord blood cells after 12- to 14-day cultures (see “Materials and methods”), and 1 × 10⁶/mL of cells in RPMI 1640 medium (FCS-free) were used for VEGF-dependent cell migration assay as indicated in panel A.