Anti-CD3ε treatment ofRAG-2^−/− thymocytes up-regulates CCR9 expression.

Total thymocytes were prepared from RAG-2–deficient animals 24, 48, and 96 hours after a single ip administration of 75 μg anti-CD3ε mAb 2C11 in PBS and stained with pAb K629 to analyze CCR9 expression (bold-line histograms). Control stainings of thymocytes from untreated RAG-2–deficient animals are also shown (dotted-line histograms). Results shown are representative of 3 independent experiments performed.