Inhibition of TF mRNA synthesis by Erk-1/2 antisense treatment.

(A) SMCs were transfected with antisense or sense oligonucleotides for 48 hours. Extracted proteins (30 μg) were separated on a 4% to 12% Bis-tris gradient gel and transferred to a PVDF membrane. Erk-1/2 was visualized by incubating the filters with a panErk-1/2 monoclonal antibody. (B) SMCs were transfected with antisense or sense oligonucleotides for 48 hours followed by stimulation with OSM (10 ng/mL) for 1 hour at 37°C. Total RNA was extracted, and TF mRNA expression was analyzed by quantitative RT-PCR. Solid bars represent the ratio of ³²P-labeled TF and s17 PCR products isolated from 2% agarose gel. Results are representative of 2 separate experiments.