TEL-Syk dimerized in COS-7 cells that required the PNT domain of TEL.

In immunoprecipitation study, COS-7 cells were cotransfected with each Flag-tagged and HA-tagged constructs: pF-TΔ PS/pH-TS (lane 1), pF-TS/pH-TS (lane 2), and pF-S/pH-TS (lane 3). Lysates were separated by SDS-PAGE, transferred to PVDF membrane, and then probed using anti-Flag mAb. Subsequently, membranes were probed using HRP-conjugated HA mAb. Cells were then lysed and immunoprecipitated with a specific antibody for Flag epitope tag. Immunoprecipitants were then separated by SDS-PAGE, transferred to PVDF membrane, and probed with HRP-conjugated anti-HA mAb.