TEL-Syk is constitutively tyrosine-phosphorylated in COS-7 cells.

COS-7 cells were transfected with pFlag-TELΔ PNT-Syk (lane 1) or TEL-Syk (lane 2), lysed, and immunoprecipitated with a mAb to Syk. Immunoprecipitants were then separated by SDS-PAGE, transferred to PVDF membrane, and blotted using HRP-conjugated tyrosine-phosphorylated antibody (PY20) and enhanced chemiluminescence (ECL) detection methods. Stripping and reprobing were performed using a mAb to Syk. Both proteins were immunoprecipitated.