TEL-Syk transforms BaF3 cells to IL-3 factor independence.

(A) BaF3 cells were infected with pBabeNeo cloned with TEL-Syk, Syk-TEL, and TELΔ PNT-Syk or vector alone and then selected for G418 resistance for 2 weeks. G418-resistant cells were washed in IL-3–free medium and plated in RPMI 1640 with 10% FCS. Viable cells were counted on each day for 5 days. (B) BaF3 cells were infected with pB-TS or pB-TΔ PS, selected, and lysed. Lysates were separated by SDS-PAGE, transferred to PVDF membrane, and incubated with a mAb to Syk. TELΔ PNT-Syk (lane 1) and TEL-Syk (lane 2) proteins were expressed in BaF3 cells.