Schematic representation of constructed Syk, TEL-Syk, Syk-TEL, and deletion mutant TELΔ PNT-Syk (deletion of nt 222-461).

For immunoprecipitation and Western blot, TEL-Syk, TELΔ PNT-Syk, and Syk cDNAs cloned into pFlag (the Flag epitope tagged) and TEL-Syk cDNA into pHM (HA epitope tagged) were used. For high expression in BaF3 cells, TEL-Syk, Syk-TEL, and TELΔ PNT-Syk were cloned into the pBabeNeo construct, a retroviral vector based on a MMLV-expressing neomycin-resistant gene. LTR indicates long-terminal repeat; SV40, SV40 promoter; SV40pA, SV40 polyadenylation signal; and CMV, CMV promoter.