Effect of vincristine treatment on the life span of mice carrying leukemias.
(A) Experimental design: CD2F1 mice were inoculated with 1.0 × 106 P388 cells, and the same number of cells was transplanted sequentially. Mice in the vincristine treatment group received a 1-mg/kg intraperitoneal dose of vincristine twice, and control mice received saline. We designated the P388 derivatives collected from the abdominal cavity of each successive group of mice as P-C0, P-C1, P-C2, P-C3, and P-C4 (untreated cells) or P-V1, P-V2, P-V3, and P-V4(vincristine-treated cells). These derivatives were used in the following experiments. (B) The life span of mice with (closed circles) or without (open circles) vincristine treatment. Arabic numbers (n = 0, 1, 2, 3, and 4) indicate the number of times that the P388 derivatives were inoculated. Mean survival days are also indicated in the lower box.
