TEL-AML1 fusion transcript as detected after serum starvation and aminophylline treatment by RT-PCR.
(A) Representative results of RT-PCR of RS4;11 cell line at various time points after serum starvation. TEL exon 5 forward primers (TEL940f and TEL981f) and AML1 exon 3 reverse primers (AML1-r31 and AML1-r32) were used in nested PCR analysis for TEL-AML1 fusion transcript. (B) Representative results of RT-PCR of RS4;11 and TS-2 cell lines at various time points after treatment with aminophylline. TELexon 5 forward primers (TEL940f and TEL981f) and AML1 exon 3 or exon 4 reverse primers (R3: AML1-r31 and AML1-r32, R4: AML1-r41 and AML1-r42, respectively) were used in nested PCR for TEL-AML1fusion transcript. H2O represents negative control in both panels A and B.
