Northern blot analysis in various individuals for the genes found to be differentially expressed between P1 and C1.

Elutriation-purified monocytes from patients with isolated Lp(a) hyperlipidemia (P1, P2), healthy controls with elevated Lp(a) (L1, L2), and 6 controls with low Lp(a) (C1 to C6) were incubated in 20% autologous serum for 18 hours, RNA was isolated, and 10 μg total RNA was used to investigate the expression of (A) MCP-1, adipophilin, CD16, C3aR, and CCR2 and (B) defensin and IP-10. Equal RNA loading was confirmed by reprobing the blots with GAPDH.