Chemoattraction of thymocyte suspensions depleted of TCRαβ⁺ cells.

Thymocyte suspensions were depleted of TCRαβ⁺ cells by the MACS system (TCRαβ⁻) as described in “Materials and methods.” (A) Migration was expressed as percentage increase of cells responding to 100 nM IP-10 versus cells migrated in the presence of medium alone. The mean values ± SE were obtained in 4 separate experiments. (B) Data from one representative experiment showing the expression of TCRγδ and CD56 by TCRαβ⁻ thymocytes migrated in response to medium alone or to 100 nM IP-10. (C) Migration of TCRγδ⁺ and CD56⁺ in response to IP-10, expressed as percentage increase in comparison with the same cells migrated in response to medium alone. The mean values ± SE obtained in 4 separate experiments are reported.