Demonstration of annexin II surface expression on RAW264.7 macrophages by flow cytometric analysis.

Control and EGTA-treated RAW264.7 macrophages were incubated with anti–annexin IgG (clone Z014, Zymed) in the absence or presence of EGTA followed by FITC-conjugated goat antimouse IgG. Relative fluorescence units are shown for each sample. To verify that annexin II was removed from the cell surface by treatment with EGTA, eluates were examined by Western blot as described in “Materials and methods” (inset).