Location of oligonucleotide primers used for allele-specific amplification of

NCF-1. Solid arrows below the gene indicate the direction and approximate position of primers used for allele-specific amplification of the p47-phox gene. Broken arrows indicate primers used for sequencing to ensure that only GTGT-containing alleles had been amplified. The 2 mutations shown above the gene, C → T in intron 1 and GTGT → GT in exon 2, are specific for ψNCF-1 (see text).