Fig. 6.
Fig. 6. Adhesion of αvβ3 mutants to immobilized fibrinogen. / WT or mutant αv construct was transiently cotransfected with WT β3 construct into 293 cells. In WT β3 cells (⋄), only WT β3 construct was transfected into cells. WT (○) or mutant αvβ3-transfected cells were incubated for 60 minutes at 37°C with immobilized fibrinogen at serial concentrations. After washing with PBS, the adherent cells were quantified with a colorimetric reaction using endogenous cellular acid phosphatase activity. ▵, Phe177Ala; ✙, Tyr178Ala; ▿, Trp179Ala; ■, untransfected cells. Data represent the mean ± SE of triplicate measures of optical density at 415 nm.

Adhesion of αvβ3 mutants to immobilized fibrinogen.

WT or mutant αv construct was transiently cotransfected with WT β3 construct into 293 cells. In WT β3 cells (⋄), only WT β3 construct was transfected into cells. WT (○) or mutant αvβ3-transfected cells were incubated for 60 minutes at 37°C with immobilized fibrinogen at serial concentrations. After washing with PBS, the adherent cells were quantified with a colorimetric reaction using endogenous cellular acid phosphatase activity. ▵, Phe177Ala; ✙, Tyr178Ala; ▿, Trp179Ala; ■, untransfected cells. Data represent the mean ± SE of triplicate measures of optical density at 415 nm.

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