Fig. 4.
Inhibition of 3H-LPS uptake in eosinophils by unlabeled LPS, compound 406, and anti-CD14 moAb.
Eosinophils were preincubated with different concentrations of unlabeled (“cold”) LPS, compound 406 (A), or anti-CD14 moAb (B) for 30 minutes at 4°C before 3H-LPS (500 ng/mL) was added. In the figure inserts, inhibition of uptake of 3H-LPS in PBMCs and monocytes by unlabeled LPS is shown. After 1 hour of incubation, cells were washed with PBS 3 times and lysis in PBS containing 2% SDS.3H-LPS counting of the lysates was performed with a liquid scintillation counter. Statistical significance versus : *P < .001.
