Inhibition of LPS-induced TNF-α and ECP release by anti-CD14 moAb and compound 406.

Eosinophils were preincubated with increasing concentrations of anti-CD14 moAb MEM-18 (A and C) and the antagonistic compound 406 (B and D) for 20 minutes at 4°C. After 16 hours of stimulation with LPS (100 ng/mL), supernatants were harvested. TNF-α content in the culture supernatant was measured by ELISA (A and B). ECP concentration was determined in radioimmunoassay (C and D).