Fig. 8.
Treatment with cytochalasin D does not correct defective adhesion of LADV cells to fibronectin.
Wells were coated overnight with 1.5 μg/well FN. LADV (░) or control (▪) lymphoblastoid cells were treated with increasing concentrations of cytochalasin D at 37°C for 15 minutes and their adhesion was measured after a subsequent 30-minute incubation on fibronectin with PMA (10−7 M). Adhesion without PMA was 2.4% for LADV cells and 17.4% for control lymphoblast cells in the absence of cytochalasin D and increased to 2.8% and 24%, respectively, with 0.1 mg/mL cytochalasin D. Adhesion to BSA in the presence of PMA was measured in parallel. Results shown are representative of at least 3 experiments.
