Fig. 3.
Chemotaxis of the patient's neutrophils was impaired.
Leukocyte-rich plasma collected from the patient (░) and from an adult control subject (▪) in parallel was placed in modified Boyden chambers and chemotaxis in response to buffer or fMLP (10−8 M) was measured after a 3-hour incubation at 37°C. The number of cells migrating completely through the filter was counted microscopically in 10 randomly selected high-power fields. The samples from the patient and control subjects were assayed in triplicate.
