Human Sn is immunoprecipitated from THP-1 cells as a single species of about 200 kd.

THP-1 cells were surface-labeled with biotin and lysates subjected to immunoprecipitation with HSN1 mAb. Samples were run either nonreduced (NR) or reduced (R) on 6.5% PAA gels, transferred to nitrocellulose, and proteins on Western blots detected with streptavidin-HRP followed by enhanced chemiluminescence. A single species is detected that migrates at about 180 kd (nonreduced) or about 200 kd (reduced).