CRP induces PI 3-kinase–dependent translocation of PLCγ2 and Btk or PI 3-kinase–independent translocation of Syk in human platelets.

Human platelets, stimulated by CRP (4 μg/mL) for 150 seconds, were fixed and stained for Syk, PLCγ2, or Btk as described in “Materials and methods” to study the protein localization. Platelets were pretreated with wortmannin (wort) 100 nM for 15 minutes before the stimulation by CRP to investigate the role of PI 3-kinase. Scale bar = 2 μm.