Overexpression of SHP2 in transfected JMC.T5 Jurkat cells enhances SDF-1α–induced migration.

(A) JMC.T5 Jurkat cells were untransfected or transfected with either control vector or SHP2 WT and then lysed with lysis buffer. Lysates (500 μg protein) from untransfected (lane 1), control vector–transfected (lane 2), or SHP2 WT–transfected (lane 3) cells were immunoprecipitated with SHP2 antibody. The immune complexes were then resolved on SDS-PAGE and blotted with SHP2 antibody. (B) The cells transfected with the control vector or SHP2 WT were subjected to migration assay with SDF-1α (50 ng/mL). The cells migrating to the bottom chamber were counted. *P < .005.