SHP2 associates with SHIP, fyn, and cbl upon SDF-1α stimulation.

Lysates obtained from Jurkat cells unstimulated (0) or stimulated with 100 ng/mL SDF-1α for various time periods were immunoprecipitated with either anti-SHIP (A), anti-SHP2 (B), anti-cbl (C), anti-SHP2 (D), the GST SH2-domain of SHP2 (E), or anti-Fyn (F). The immune complexes were then run on SDS-PAGE and immunoblotted with anti-SHP2 antibody (A,C,F) or anti-SHIP (B) or anti-cbl antibody (D,E). C represents the antibody control, and TCL is 50 μL of total cell lysate.