CXCR4 associates with SHP2 upon SDF-1α stimulation.

Lysates obtained from Jurkat cells unstimulated (0) or stimulated for varying time periods with 100 ng/mL SDF-1α were immunoprecipitated with CXCR4. The immune complexes were subjected to SDS-PAGE and immunoblotted with anti-SHP2 antibody. C represents the antibody control, and TCL is 50 μL of the total cell lysate.