Tyrosine phosphorylation of SHP2 upon SDF-1α stimulation.

CXCR4 L1.2 (A) or Jurkat (B) cells were either unstimulated (0) or stimulated with 100 ng/mL SDF-1α for varying time periods. Cells were then lysed and immunoprecipitated with anti-SHP2 antibody. The immune complexes were run on SDS-PAGE and immunoblotted with antiphosphotyrosine antibody (top panels), followed by anti-SHP2 antibody (bottom panels). C represents the antibody control, and TCL is 50 μL of the total cell lysate. IP indicates immunoprecipitation; WB, Western blot.