Determination of telomere length in MHC class I/peptide tetramer staining on PBMCs isolated from AIM patients.

(A) PBMCs from an AIM patient stained with CD8 RPE-Cy5 and HLA-A2 GLC-PE tetramer. Cells were gated to exclude doublets and dead/dying cells as determined by propidium iodide (Figure 1B). At least 50 000 live events were acquired on the flow cytometer. (B) Two-color flow-FISH analysis of telomere staining (FITC) of A2-GLC tetramer–positive cells (A2-GLC–Cy5) in an acute AIM patient. At least 50 000 live events were acquired.