Fig. 4.
Results showing that growth factor–independent G0 cells are exclusively
BCR-ABL+. RT-PCR analysis ofBCR-ABL and actin expression was done on 20 clones derived from single, initially quiescent CD34+ cells (10 each from patients 1 and 2) after culture for 10 days in the absence of added growth factors. PCR products were hybridized by using complementary DNA probes specific for BCR-ABL and actin, respectively. Con 1, 1000 CML cells; con 2, 1000 normal bone marrow cells; con 3, 1000 CML cells without reverse transcriptase; con 4, water.
