Mutated sites in the human fVIII C2 domain.

(A) Ribbon diagram showing hydrophobic residues proposed to be involved in phospholipid membrane binding and Lys2227, 1 of the 4 putative positively charged binding residues.11 Met2199, Phe2200, Val2223, Lys2227, and Leu2252 were mutated in this study. Leu2251, which is conserved in human, porcine, murine, and canine fVIII, was not mutated. (B) Space-filling model rotated, as if looking up from the membrane.