Genomic PCR for the Flt3/ITD.

Genomic DNA prepared from diagnostic marrow specimens were PCR amplified and resolved on polyacrylamide gel. C, non-nucleic acid control; NL, normal marrow control; 1-9, samples from 9 different pediatric patients. High-molecular weight bands in lanes 4 and 7 represent the Flt3/ITD. Patient sample in lane 4 does not have a normal Flt3 band. This patient was previously shown to have a loss of heterozygosity (LOH) of chromosome 13 at the region of theFlt3 gene. Arrow points to the normal Flt3 gene product.