Immunostimulatory capacity of generated LCs.

CD1a⁺ LCs were generated in primary cultures and further stimulated in cytokine-supplemented medium (TGF-β1 plus GM-CSF, TNFα, SCF, and FL, ■) or identical medium containing anti–E-cadherin mAb (E-cad, ○) or control mAb (clone VIAP, ⋄), as described in “Materials and methods” (and as also shown in Figure8). Graded numbers of cells obtained from these cultures after 24 hours were used to stimulate 5 × 10⁴ purified allogeneic T cells (see “Materials and methods”). Error bars represent standard deviation of triplicate values. Data are representative of 3 experiments.