Effects of mAb addition on maturation of purified CD1a⁺ LCs.

CD34⁺ cells were cultured in the presence of GM-CSF plus TNFα, SCF, FL, and TGF-β1 in serum-free medium (see “Materials and methods”). CD1a⁺ cells from these 1° cultures generated after 10 to 12 days were purified by flow sorting and replated in 2° cultures at a high cell density and in the presence or absence of mAbs to adhesion molecules as indicated (see “Materials and methods”). Diagrams show CD86 versus CD1a molecule expression of cells from representative 2° cultures (after 24 hours of culture) supplemented with mAbs, as indicated. Markers were set according to isotype-matched negative control staining (n = 4).