Representative cell morphology in the presence of mAbs to adhesion molecules.

Generated cells from primary LC generation cultures were harvested and replated at a high cell density in 2° cultures supplemented with the cytokines GM-CSF plus TNFα, SCF, FL, and TGF-β1 in the presence or absence of mAbs specific for adhesion molecules. Photomicrographs show representative cell morphology (original magnification, ×20) of these 2° cultures before (B) or after (A, C, D) culture for 24 hours. Monoclonal antibodies of the following specificities were added: A, control; C, E-cadherin; D, CD43 (see also “Materials and methods”).