Fig. 5.
Addition of TGF-β1 to serum-free 2° cultures does not inhibit the up-regulation of CD86 or CD83 expression.
Cells were generated and replated in 2° cultures as described in Figure 2, and 2° cultures were supplemented with GM-CSF plus TNFα or GM-CSF plus CD40L. The effect of the addition of TGF-β1 to these 2° cultures on the expression of CD86 or CD83 by cultured cells is shown. Representative histograms show CD86 or CD83 expression of cells stimulated in 2° cultures in the presence or absence of TGF-β1, as indicated.
