Fig. 1.
Fig. 1. Cells generated in the presence of TGF-β1 show specific features of immature epidermal LCs. / (A) Purified CD34+ cells were cultured for 14 days in defined serum-free medium supplemented with the cytokines GM-CSF plus TNFα, SCF, and FL in the presence or absence of TGF-β1. Day 14–generated cells were harvested and analyzed for the expression of informative molecules. Diagrams show the correlated expression of CD1a versus CD86, CD83, or E-cadherin, respectively, by generated cells in the presence or absence of TGF-β1. (B) Time kinetics analysis of cells generated in cultures of CD34+ cells supplemented with the cytokines TGF-β1 plus GM-CSF, TNFα, SCF, and FL. Diagrams show combined staining of parallel cultures harvested at day 7 or 16 for CD1a versus CD86 or CD83 expression, respectively. Markers were set according to negative control staining.

Cells generated in the presence of TGF-β1 show specific features of immature epidermal LCs.

(A) Purified CD34+ cells were cultured for 14 days in defined serum-free medium supplemented with the cytokines GM-CSF plus TNFα, SCF, and FL in the presence or absence of TGF-β1. Day 14–generated cells were harvested and analyzed for the expression of informative molecules. Diagrams show the correlated expression of CD1a versus CD86, CD83, or E-cadherin, respectively, by generated cells in the presence or absence of TGF-β1. (B) Time kinetics analysis of cells generated in cultures of CD34+ cells supplemented with the cytokines TGF-β1 plus GM-CSF, TNFα, SCF, and FL. Diagrams show combined staining of parallel cultures harvested at day 7 or 16 for CD1a versus CD86 or CD83 expression, respectively. Markers were set according to negative control staining.

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