Presence of membrane-bound BLyS on human PBMNCs.

We performed 2-color staining on freshly purified cells using FITC-coupled antibodies specific for CD3 (T lymphocytes), CD20 (B lymphocytes), CD56 (NK cells), and CD14 (monocytes). Presence of BLyS on the cell surface was detected by biotinylated 9B6 followed by PE-conjugated streptavidin. PBMNCs from 6 donors were analyzed for BLyS expression. A representative experiment is shown.