American Society of Hematology

Fig. 5.

$Fig. 5. Hst/Py staining of adult BM CD34+ cells before and after culture with growth factor combination A. / Lin− adult BM cells were labeled with CD34-FITC, Hst and Py as described in the “Materials and methods,” and then the gates shown were used to isolate the G0(HstloPylo) fraction (middle panel) from within the PI− CD34+ population (left panel). These G0i cells were then cultured in serum-free medium containing FL, SF, IL-3, IL-6, and G-CSF (Comb. A) for 40 hours. The cells were then restained as before to allow the isolation of the persisting G0 cells (G0′) by FACS separate from the remaining G1/S/G2/M (NG0′) cells.$

Hst/Py staining of adult BM CD34⁺ cells before and after culture with growth factor combination A.

Lin⁻ adult BM cells were labeled with CD34-FITC, Hst and Py as described in the “Materials and methods,” and then the gates shown were used to isolate the G₀(Hst^loPy^lo) fraction (middle panel) from within the PI⁻ CD34⁺ population (left panel). These G₀i cells were then cultured in serum-free medium containing FL, SF, IL-3, IL-6, and G-CSF (Comb. A) for 40 hours. The cells were then restained as before to allow the isolation of the persisting G₀ cells (G₀′) by FACS separate from the remaining G₁/S/G₂/M (NG₀′) cells.

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