Time course of changes in gene expression in variously growth factor-stimulated primitive adult BM cells.

Panel A shows the Southern blot analyses of transcript cDNAs detected in 2 independent experiments in which FACS-sorted CD34⁺CD38⁻ cells were stimulated with one of 5 different growth factor conditions (as shown) and the cells harvested for RT-PCR analysis at the times indicated. Panel B shows the same data normalized as described in the “Materials and methods” using the corresponding starting CD34⁺CD38⁻ BM cells as the reference population in each case (values set = 1.0). Each symbol identifies the results obtained for a different growth factor condition (●, TPO; ■, Comb. A; □, Comb. B; ▴, Comb. C; Δ, Comb. D).