Proviral integration in myeloid and B-lymphoid cells from Bcr-Abl/ΔSH2 mice.
(A) Genomic DNAs of PB cells of mouse BMT11.48 (lane 3) and its secondary recipients BMT11.48.36 (lane 4) and BMT11.48.38 (lane 5), sorted myeloid (lane 1) and B-lymphoid (lane 2) cells of Bcr-Abl/ΔSH2 mouse BMT11.34, and sorted myeloid (lane 7) and B-lymphoid (lane 8) cells of Bcr-Abl/ΔSH2 mouse BMT11.53 were digested with restriction enzyme EcoRI and analyzed by Southern blotting with32P-labeled IRES-gfp sequences. Common bands (same size) in B-lymphoid cells and myeloid cells from the same mouse are labeled with an asterisk (lanes 1,2) for BMT11.34 and an arrowhead (lanes 7,8) for BMT11.53. Sizes of HindIII-digested λ DNA fragments were used as DNA molecular weight markers and are shown on the right. (B) DNA from mice BMT11.48.36 and BMT11.48.38 were digested withBamHI (lanes 1,2) and BglII (lanes 3,4) and analyzed by Southern blot with the same probe as in panel A. Standard 1-kb size marker is shown partially on the right.
