Aggregation response of platelets to chemokines alone and in presence of ADP receptor inhibitor AR-C66096 or ADPase.
In the upper left series, washed platelets (5 × 108/mL) were treated with 100 nmol/L TARC, RANTES, SDF-1, or MDC, respectively. In the upper right series, washed platelets (5 × 108/mL) were treated with a mixture of 100 nmol/L each TARC plus SDF-1 or RANTES plus TARC, respectively. No signal addition was seen when the same doses were given successively (Figure4). In the lower 2 aggregation profiles, washed platelets were treated with 100 nmol/L TARC in the presence of 100 nmol/L ADP receptor inhibitor (AR-C66096) or (bottom profile) with 100 nmol/L RANTES in the presence of 5 μmol/L apyrase. The results shown here are representative of at least 3 similar experiments.
