Fig. 4.
Platelet cytoplasmic Ca++ response to various chemokines measured using fura-2 fluorescence.
Washed platelets (108/mL) were loaded with fura-2 and then activated with 100 nmol/L chemokine. When the signal had returned to baseline or stabilized (generally at 2 minutes) either a second dose of the same chemokine or of another one was added. Partial mutual desensitization of the Ca++ signal response by chemokines using different receptors was observed but was almost complete when the same chemokine was used twice or when chemokines with the same receptor were used. The results shown are typical of at least 3 similar experiments.
