Expression of CCR1 and CCR4 in platelets assessed by immunoprecipitation and immunoblotting using antipeptide polyclonal antibodies.

Left panel, detection with anti-CCR1 polyclonal antibodies; 1, platelet lysate; 2, immunoprecipitate from platelets of donor A with anti-CCR1 polyclonal antibodies; 3, immunoprecipitate from platelets of donor B with anti-CCR1 polyclonal antibodies; 4, immunoprecipitate from monocytes with anti-CCR1 polyclonal antibodies. Right panel, detection with anti-CCR4 polyclonal antibodies; 1, platelet lysate; 2, immunoprecipitate from platelets of donor A with anti-CCR4 polyclonal antibodies; 3, immunoprecipitate from platelets of donor B with anti-CCR4 polyclonal antibodies; 4, immunoprecipitate from CCR4 transfected cells with anti-CCR4 polyclonal antibodies.