Expression of laminin chains in human bone marrow.

The micrographs show immunofluorescence stainings of bone marrow cryostat sections stained with the following antilaminin antibodies: (A) antiserum against the α4 chain; the staining is mainly found in intersinusoidal spaces; (B) mAb 4C7 against the α5 chain; expression is restricted to the endothelial cells; (C) mAb against the β1 chain; (D) mAb C4 against the β2 chain; the staining is restricted to the larger blood vessels; (E) mAb D18 against the γ1 chain; and (F) an antiserum against the LN-1 isoform recognizing α1, β1, and γ1 chains. Comparable staining patterns in intersinusoidal spaces and in basement membranes of adipocytes and endothelial cells are apparent in panels C, E, and F, although at different intensities. (Original magnification × 110.)