Immunofluorescence stainings of LTMCs.

The adherent stromal layer of human LTMCs were stained with an antilaminin α4 chain antiserum (A); the antibody, 4C7, recognizing the α5 chain (B); the antibody, C4, against the β2 chain (C); or an antiserum against the LN-1 isoform, recognizing α1, β1, and γ1 chains (D). All 4 antibodies show strong staining signals. Note that the staining signals were restricted to the cytoplasmic part of the cells, and no extracellular deposition could be detected. (Original magnification × 270.)