American Society of Hematology

Fig. 6.

$Fig. 6. RNA blot analysis of CCR8 mRNA in HUVECs. / Aliquots (10 μg) of total RNA from Jurkat cells (Ju) or from confluent HUVECs (Hu) were size-fractionated on agarose gels and hybridized to 32P-labeled human CCR8 cDNA as described in “Materials and methods.” GAPDH (GAP) is shown as a control for loading of samples. The cDNA was cloned as detailed in “Materials and methods” and the identity of the fragment as CCR8 mRNA was confirmed by sequence analysis.$

RNA blot analysis of CCR8 mRNA in HUVECs.

Aliquots (10 μg) of total RNA from Jurkat cells (Ju) or from confluent HUVECs (Hu) were size-fractionated on agarose gels and hybridized to ³²P-labeled human CCR8 cDNA as described in “Materials and methods.” GAPDH (GAP) is shown as a control for loading of samples. The cDNA was cloned as detailed in “Materials and methods” and the identity of the fragment as CCR8 mRNA was confirmed by sequence analysis.

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