Fig. 6.
Tyrosine 155 sulfation of myotube-synthesized and Hep G2 cell-synthesized F.IX.
(A) 35S-H2SO4 labeling. (B)35S-methionine labeling of synthesized recombinant FIX. Lanes 1 and 3 are myotube-synthesized F.IX. Lanes 2 and 4, HepG2 synthesized F.IX. Lanes 3 and 4, F.IX after digestion with N-glycosidase F. Lanes 1 and 2, undigested proteins. (C) Western blot of the same gel using a monoclonal antibody to heavy chain of hF.IX demonstrated that the band shown in panel A is human F.IX.
