Fluorophore-assisted carbohydrate electrophoresis (FACE) profiling of sialic acid residues.

(Lane 1) Wheat starch digest control yielding glucose polymers ranging from G3 (lowest band in lane 1) to approximately G15. In lanes 2 to 5, equal amounts of labeled oligosaccharide profile mixtures are loaded after a 24-hour incubation either with (lanes 3, 4) or without (lanes 2, 5) neuraminidase. (Lanes 2, 3) Myotube-synthesized F.IX. (Lanes 4, 5) Human plasma-derived F.IX.