Antiadhesive function of the 130-kd CD43 glycoform on T cells.
T-cell clones (A) from B/WF1 mice positive for MFT3 antigen (KGU139, ■) and negative for MFT3 antigen (KGU140, ░) and T-cell lines (B) from BALB/c mice positive for MFT3 antigen (SA-3, ■) and negative for MFT3 antigen (line 8-3, ░) were used for adhesion assays. T-cell lines or clones were radiolabeled with chromium 51 (51Cr) and cultured with spleen cells, M12.C3 cells, M12.4.5 cells, or BALB/3T3 (3T3) cells as adhesion target-cell monolayers. After 30 minutes, nonadherent cells were removed and the radioactivity of adherent-cell–associated 51Cr was determined. The percentage of adhesion was calculated as the counts per minute of adhered cells divided by the counts per minute of input cells times 100. (C) Autoreactive T-cell clones KGU139 (♦) and KGU140 (▴) derived from B/WF1 mice and KLH-reactive SA-1 (○), SA-2 (+), SA-3 (×), line 3 (●), line 8-3 (▪) T-cell lines derived from BALB/c mice were stained with MFT3 mAb and analyzed on a FACScan. The T-cell lines and clones were assayed for cell adhesion by using various adhesion target cells at the same time as MFT3 mAb staining. Individual T-cell clones were assayed repeatedly at different times. Results were expressed as the percentage of adhesion compared with MFT3 expression (as a mean fluorescence intensity ratio of MFT3 staining to control staining).
