C2GnT transcripts were detected in MFT3-positive but not MFT3-negative cells on analysis with RT-PCR.

(A) Total RNA from MFT3 antigen–positive (KGU139) and MFT3 antigen–negative (KGU140) T-cell clones was subjected to RT and amplified by PCR using C2GnT-specific primers or β-actin primers. (B) Total RNA from 293 cells and BALB/3T3 cells that were used as host cells in transfection experiments was subjected to RT-PCR analysis using the primers as in Figure 4A. PCR products were electrophoresed in agarose gels and stained with ethidium bromide. RT (+) indicates RNA reverse transcribed; and RT (−), RNA not reverse transcribed.